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2 edition of Studies on thermally-induced DNA damage in Streptococcus faecalis found in the catalog.

Studies on thermally-induced DNA damage in Streptococcus faecalis

J. P. Greaves


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Studies on thermally-induced DNA damage in Streptococcus faecalis by J. P. Greaves Download PDF EPUB FB2

Germ-free interleukin knockout (IL KO) mice developed inflammatory bowel disease (IBD) after they were colonized with a pure culture of Enterococcus faecalis.E. faecalis not only induced IBD (primarily in colon and rectum) but rectal dysplasia and adenocarcinoma was also found in the IL KO mice.

Conventional (complex-intestinal flora) IL KO mice developed IBD within 10 to 15 weeks Cited by: The cell-free DNA (cf-DNA) concentrations in plasma of rats were determined by using Quant-it Picogreen double-stranded DNA (dsDNA) reagent (Invitrogen, France). Briefly, μl amounts of samples and Lambda DNA standard (1 ng/ml to 1 μg/ml) were diluted in Tris EDTA buffer ( mM Tris-HCl, 20 mM EDTA, pHμl final amount) before the Cited by: Studies on thermally-induced DNA damage in Streptococcus faecalis Author: Greaves, J.

ISNI: Awarding Body: Leicester Polytechnic Current Institution: De Montfort University Date of Award: Availability of Full Text. Few studies have utilized this method to investigate the microbiota of endodontic infections (11, 12). This study focused on the prevalence of Actinomyces species, Streptococcus species, and E.

faecalis in primary root canal infections as evaluated by the checkerboard DNA-DNA hybridization assay. MATERIALS AND METHODS Specimen SamplingCited by: ENTEROCOCCI AND THE GENUS ENTEROCOCCUS. Previously classified in the genus Streptococcus, the enterococci were proposed to be a division comprised of bacteria that generally grow at temperatures of between 10°C and 45°C in % NaCl at pH and to survive at 60°C for 30 min (66, 68,).This classification scheme, proposed previously by Sherman (), correlated with a Cited by: Concentration.

A low concentration of sperm can be a result of genital tract infection. For example, studies showed that the presence of P. aeruginosa or S. faecalis in the seminal fluid could lead to a reduction in sperm concentration [21,65].It also has been reported that the genital tract infection by Enterococcus faecalis (E.

faecalis) or CT is associated with compromised sperm. Streptococcus faecium E showed a D value of min at this temperature and Streptococcus faecalis P-2A was the least heat resistant organism with a D value of min at 66°C. It was demonstrated that these three organisms showed greater thermal resistance in the raw ham than in Sorenson's buffer or ham broth, as determined in a.

Enterococcus faecalis is a Gram-positive minority constituent of the colonic microbiota that can directly damage epithelial cell DNA (Huycke et al., ), promote chromosomal instability through a macrophage-induced bystander effect (Wang & Huycke, ), and trigger colitis and cancer in interleukin (IL) knockout mice (Balish & Warner.

Identification of E. faecalis and E. faecium. A µL multiplex PCR was used to detect the sodA gene in positive Enterococcus species. The primer sequence (Table 2) used to detect the sodA gene, of bp and bp for E. faecalis and E. faecium, respectively, is depicted in Table reaction mixture contained µL of the 10X master mix, µL of sterile water, 1 pmol of each.

Certain strains of streptococci have been shown to be capable of conjugatively transferring drug resistance determinants in the apparent absence of plasmid DNA The transferrable tetracycline resistance (Tc r) marker of Streptococcus faecalis DS16 has been located on a transposon, designated Tn 2,4,7.

We show here that when chromosomal Tn is conjugatively transferred it inserts. Microbial infections can happen on a daily basis. There are specific microbial infections that are associated with the reproductive health.

Infertility is a devastating health problem. It is estimated that out of 15% of infertility worldwide, around 50% is due to male partner. Infections due to Chlamydia trachomatis, Mycoplasma genitalium, hepatitis B virus, tuberculosis, Streptococcus.

If the culture is an unidentified gram-positive coccus, an Enterococcus, viridans Streptococcus, or of unknown identity (basically includes all cultures other than pneumococci, ß-hemolytic streptococci, and.

UntilE. faecalis was known as Streptococcus ists previously categorized the bacteria as part of the genus Streptococcus. According. Streptococcus pyogenes is a Group A Streptococcus species that is non-spore forming, Gram-positive, non-motile round to ovoid bacterium that occur in chains, singly (as coccus) or in pairs (as diplococci).

It is the most common cause of pharyngitis (sore throat) in humans. Other streptococcal diseases caused by Streptococcus species in humans include scarlet fever, cellulitis, necrotizing.

Streptococci originally designated as S. mutans were isolated from dental caries and from bacterial endocarditis. In contrast to the initially perceived phenotypic homogeneity, subsequent serological, genetic, and biochemical studies showed considerable heterogeneity (Table 1).

16S rRNA data place Streptococcus criceti, Streptococcus dentapri, Streptococcus dentirousetti, Streptococcus. A global rise in antimicrobial-resistant infections has resulted in increased morbidity and mortality for patients of all ages and backgrounds [1, 2].The Centers for Disease Control and Prevention estimate that more than million antibiotic-resistant infections occur annually in the United States, accounting for more than 35 deaths [].

More recently, nucleic acid studies (7, 12) have confirmed that Streptococcus faecalis and Streptococcus faecium are only distantly related to Streptococcus bovis and Streptococcus equinus.

Enterococcus faecalis. It was the only possible member of the Streptococcaceae family that ferments Mannitol. All additional biochemical tests confirmed the identification of the unknown and no problems were encountered.

Enterococcus faecalis is a gram positive bacterium that inhabits the gastrointestinal tracts of humans or other mammals. and putrescine was evaluated by PCR for Streptococcus equines FAA and Enterococcus faecalis FAA To detect the tyrosine decarboxylase gene, (tdc), Ten (10 ng) of extracted DNA was diluted in 25 ml of MilliQ water and used directly in the reaction.

DNA was amplified in an iCycler thermal cycler (Bio-Rad, Hercules, Calif.). The PCR. Formerly known as Streptococcus faecalis and Streptococcus faecium(1). CHARACTERISTICS: Enterococcus spp. are facultatively anaerobic, catalase-negative Gram- positive cocci, arranged individually, in pairs, or short chains(1,2).

Optimal temperature for growth of E. faecalis. The present study was concerned with bacterial myocarditis where it was carried out on four groups of rats, each group is composed of ten rats (n= 10).

Three of the four groups were injected by Streptococcus faecalis intra- peritoneally and the fourth group was treated as control group and was injected by saline. All rats in the experimental.A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text.Genomic DNA isolation, PCR, and DNA sequencing.

E. faecalis isolates freshly streaked from freezer vials onto brain heart infusion agar (Difco Laboratories, Detroit, Mich.) were cultured in brain heart infusion broth. Genomic DNA was extracted by the hexadecyltrimethyl ammonium bromide method as described previously ().The ef numbers used in this study are from the V genome annotation ().